Table 1.
Predicted function of ORFs from plm gene cluster for a putative polyene macrolactam (pretilactam, 1) in ATCC 31565.
| ORF | Size (aa) | Homologue [Accession No./Strain] | Identity/Positive | Predicted Function |
|---|---|---|---|---|
| plm1 | 210 | TetR/AcrR family [ACU36614.1/Actinosynnema mirum DSM 43827] | 92/96 | transcriptional regulator |
| plm2 | 613 | major facilitator superfamily MFS_1 [ACU36615.1/Actinosynnema mirum DSM 43827] | 95/96 | MFS transporter |
| plmA | 1816 | short-chain dehydrogenase/reductase SDR [ACU36616.1/Actinosynnema mirum DSM 43827] | 97/98 | type I modular PKS |
| plmB | 3092 | short-chain dehydrogenase/reductase SDR [ACU36617.1/Actinosynnema mirum DSM 43827] | 96/97 | type I modular PKS |
| plmC | 1606 | short-chain dehydrogenase/reductase SDR [ACU36618.1/Actinosynnema mirum DSM 43827] | 96/97 | type I modular PKS |
| plmD | 5400 | short-chain dehydrogenase/reductase SDR [ACU36619.1/Actinosynnema mirum DSM 43827] | 96/97 | type I modular PKS |
| plmE | 296 | proline-specific peptidase [ACU36620.1/Actinosynnema mirum DSM 43827] | 96/97 | peptidase |
| plmF | 82 | phosphopantetheine-binding protein [ACU36622.1/Actinosynnema mirum DSM 43827] | 100/100 | phosphopantetheine-binding protein |
| plmG | 529 | AMP-dependent synthetase and ligase [ACU36623.1/Actinosynnema mirum DSM 43827] | 96/97 | synthetase and ligase |
| plmH | 507 | AMP-dependent synthetase and ligase [ACU36624.1/Actinosynnema mirum DSM 43827] | 98/98 | synthetase and ligase |
| plmI | 308 | S-malonyltransferase-like protein [ ACU36625.1/Actinosynnema mirum DSM 43827] | 98/98 | ACP S-malonyl transferase |
| plmJ | 419 | pyridoxal phosphate-dependent aminotransferase [ACU36626.1/Actinosynnema mirum DSM 43827] | 97/98 | aminotransferase |
| plmK | 450 | l-lysine 2,3-aminomutase [ACU36627.1/Actinosynnema mirum DSM 43827] | 99/99 | glutamate 2,3-aminomutase |
| plmL | 395 | cytochrome P450 [ACU36628.1/Actinosynnema mirum DSM 43827] | 98/99 | cytochrome P450 oxidase |
| plmM | 7110 | short-chain dehydrogenase/reductase SDR [ACU36630.1/Actinosynnema mirum DSM 43827] | 97/98 | type I modular PKS |
| plm3 | 931 | transcriptional regulator, LuxR family [ACU36631.1/Actinosynnema mirum DSM 43827] | 98/98 | transcriptional regulator |
| plm4 | 279 | ABC transporter related [ACU36632.1/Actinosynnema mirum DSM 43827] | 99/100 | ATP-binding protein |
| plm5 | 855 | FtsX-like permease family protein [ACU36633.1/Actinosynnema mirum DSM 43827] | 98/99 | FtsX-like permease family |