Fig. 1.

Evaluating transgenerational transfer of adoptively transferred T cells. (1) T cells are isolated from splenocytes and transduced to express a transgenic CAR. (2) Female mice are tumor challenged and treated with CAR T cells, then followed for tumor resolution. (3) After varying intervals, these females or wild type females are bred to have offspring which are (4) then cross fostered. (5) Pups are weaned and challenged with tumor cells or not, then evaluated for tumor growth, autoimmune responses and persistence of transgenic T cells