Table 1.
Hydration volume (mL), hydration time (min), niosome size (nm) pre-sonication and post-sonication, and polydispersity index (PDI) pre-sonication and post-sonication of methylene blue-loaded niosomal formulations.
| Formulation a | Hydration Volume (mL) | Hydration Time (min) | Niosome Size (nm) | PDI | ||
|---|---|---|---|---|---|---|
| Before Sonication | After Sonication | Before Sonication | After Sonication | |||
| E1 S60: Cho: ELP b |
20 | 60 | 804.0 ± 294.8 | 272.9 ± 105.1 | 0.56 ± 0.28 | 0.39 ± 0.24 |
| E2 S60: Cho: ELP b |
5 | 15 | 1514.3 ± 203.5 | 292.4 ± 12.3 | 0.40 ± 0.17 | 0.38 ± 0.23 |
| E3 S60: Cho: ELP b |
5 | 60 | 615.7 ± 126.8 | 362.2 ± 138.3 | 0.71 ± 0.25 | 0.45 ± 0.17 |
| E4 S60: Cho: ELP b |
2.5 | 60 | 619.7 ± 191.2 | 311.7 ± 27.4 | 0.72 ± 0.16 | 0.36 c 0.16 |
| L1 S60: Cho: L90 b |
5 | 60 | 1463.4 ± 62.4 | 256.9 ± 51.9 | 0.46 ± 0.06 | 0.34 ± 0.13 |
For all experiments, results were the average of triplicates from three prepared lots (n = 3) ± standard deviation. a All formulations E1, E2, E3, E4, and L1 were prepared at a molar ratio of 45:45:10. b Span® 60 (S60); cholesterol (Cho); Cremophor® ELP (ELP); Lauroglycol® 90 (L90).