Table 3.
Retention times (RT), MS data, and tentatively identified compounds in the E. japonica extract.
| RT (min) | [M-H]− (m/z) | MS/MS (m/z) | PDA λmax (nm) | Tentative Identification | References |
|---|---|---|---|---|---|
| 11.05 | 352.96 | 110.40; 143.67; 179.20; 191.20; 284.50; 312.26 | 234; 295; 325 | probably chlorogenic acid | [41] |
| 420.95 | 259.61; 301.23; 331.19; 343.20; 352.64; 360.36; 375.14; 385.20; 392.55; 403.15 | n.c. | |||
| 17.18 (16.48–17.26) | 463.17 | 151.00; 179.09; 190.34; 221.17; 255.30; 271.50; 300.25; 301.16; 325.03; 343.10; 373.35; 400.93; 418.54; 445.14 | 234; 347 | hyperoside or isoquercetin isomers | [42,43] |
| 547.19 | 220.44; 292.64; 310.81; 384.91; 437.90; 478.82; 500.58; 515.88 | n.c. | |||
| 593.04 | 255.34; 284.19; 327.19; 411.21; 429.21; 447.18; 473.09; 565.32 | n.c. | |||
| 855.43 | 417.31; 545.16; 563.31; 691.43; 709.34; 735.38; 864.27 | n.c. | |||
| 901.14 | 299.82; 439.19; 610.71; 721.14; 763.80; 854.33; 914.59 | n.c. | |||
| 28.12 | 821.37 | 511.74; 529.60; 657.60; 675.39; 721.14; 766.16 | 221; 234; 280 | (trans)nerolidol-trirhamnopyranosyl-glucopyranoside or loquatifolin A | [44] (compound 1 or 4) |
| 867.12 | 596.27; 675.92; 690.31; 721.42; 740.66; 786.99; 815.64; 820.05; 833.88 | n.c. | |||
| 1688.98 | 551.69; 696.87; 719.35; 821.28; 865.28; 881.05; 1275.89; 1396.00; 1541.66 | n.c. | |||
| 29.11 | 807.37 | 529.33; 661.43; 675.31 | 217; 234; 280 | unknown new compound | [44](compound 2) |
| 853.02 | 350.34; 454.48; 503.33; 649.33; 731.12; 784.53; 809.49; 839.42; 853.53 | n.c. | |||
| 31.52 | 675.31 | 204.97; 307.09; 383.17; 467.33; 529.20; 574.81 | 221; 234; 283; 312 | nerolidol-dirhamnopyranosyl-glucopyranoside | [44] (compound 3 or 5) |
| 721.21 | 490.37; 597.16; 675.29 | [M+HCOO-H]− of 675.31 [M-H]− | [44] | ||
| 33.12-33.67 | 967.47 | 309.10; 351.25; 395.15;437.27; 511.25; 529.48; 579.22; 639.41; 657.34; 675.47; 717.98; 743.29; 761.35; 803.44; 821.38; 848.32; 865.41; 922.75; 945.31 | 334; 289; 323 | n.c. | |
| 997.51 | 381.23; 467.03; 511.14; 529.05; 543.22; 567.25; 603.04; 657.15; 675.18; 697.48; 721.08; 773.26; 803.34; 821.36; 833.47; 915.25; 938.24 | probably nerolidol--rhamnopyranosyl -rhamnopyranosyl--(4-trans-feruloyl)-rhamnopyranosyl--glucopyranoside | [45] | ||
| 1065.21 | 405.44; 513.91; 579.73; 675.96; 1020.72; 1033.32; 1041.27; 1057.41; 1067.28 | n.c. | |||
| 50.02 | 633.52 | 339.61; 469.47; 487.39; 513.48; 571.44; 589.50; 615.47; 633.50 | 219; 235; 310 | probably 3-O-p-coumaroyltormetic acid | [46] |
| 1267.27 | 1102.55 | n.c. | |||
| 62.90 | 523.39 | - | 219; 235; 281 | Usolic acid (monomer adduct)* | Standard |
| 933.70 | 408.37; 455.50; 500.98; 584.57; 745.53; 870.99; 933.70 | Usolic acid [2M + Na+ -2H+]− | Standard | ||
| 1411.83 | 455.52; 501.44; 933.71; 1302.93; 1377.25; 1410.15 | Usolic acid [3M + 2Na+ -3H+]− | Standard | ||
| 1885.16 | 934.78; 1391.11; 1406.28; 1447.24; 1608.90; 1743.24; 1855.24 | Usolic acid [4M + 3Na+ -4H+]− | Standard |
All further mass peaks were assumed to be chlorophyll related, because of absorption maxima of 408 nm and higher. n.c.: not classifiable. Fragments shown in bold are the main fragments.* Ursolic acid shows, instead of its monomer ion (m/z 455.50 [M-H+]−), an unknown adduct combination (X) with m/z 523.39 [M+X-H+]− and forms additional dimer, trimer, and tetramer adducts with Na+ ions.