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. 2019 May 30;6(2):61. doi: 10.3390/medicines6020061

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© 2019 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Schematic representation of zebrafish bioassay-guided isolation of natural products. (A) Different sources of natural products. (B) Crude extraction of natural products. (C) Purification of biologically active compounds from various chromatographic methods. (D) Various zebrafish biological assays and structural characterization of HIT compounds using different spectroscopic techniques. (HPLC: high performance liquid chromatography; MS: mass spectrometry; NMR: nuclear magnetic resonance spectrometry; NPs: natural products; and TLC: thin-layer chromatography).