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. 2019 Jun 7;8(6):159. doi: 10.3390/plants8060159

Table 4.

Advanced development of the quantitative methods by HPLC for MA and MB.

Parameters This Study Previous Study [13]
Sep-Pak C18 cartridge Used Not use
HPLC column 10 µm, 150 mm × 4.6 mm i.d. 10 µm, 250 mm × 4.6 mm i.d.
Flow rate 0.5 mL/min 0.4 mL/min
LOD 0.05 ng/mL (MA), 0.48 ng/mL (MB) 0.43 ng/mL (MA), 0.18 ng/mL (MB)
LOQ 0.14 ng/mL (MA), 1.46 ng/mL (MB) 1.31 ng/mL (MA), 0.54 ng/mL (MB)
Quantity of MA 51.96 µg/mL 16.44 µg/mL
Quantity of MB 42.33 µg/mL 9.24 µg/mL

HPLC, high performance liquid chromatography; LOD, limit of detection; LOQ, limit of quantification; MA, momilactone A; MB, momilactone B.