Table 4.
Parameters | This Study | Previous Study [13] |
---|---|---|
Sep-Pak C18 cartridge | Used | Not use |
HPLC column | 10 µm, 150 mm × 4.6 mm i.d. | 10 µm, 250 mm × 4.6 mm i.d. |
Flow rate | 0.5 mL/min | 0.4 mL/min |
LOD | 0.05 ng/mL (MA), 0.48 ng/mL (MB) | 0.43 ng/mL (MA), 0.18 ng/mL (MB) |
LOQ | 0.14 ng/mL (MA), 1.46 ng/mL (MB) | 1.31 ng/mL (MA), 0.54 ng/mL (MB) |
Quantity of MA | 51.96 µg/mL | 16.44 µg/mL |
Quantity of MB | 42.33 µg/mL | 9.24 µg/mL |
HPLC, high performance liquid chromatography; LOD, limit of detection; LOQ, limit of quantification; MA, momilactone A; MB, momilactone B.