Table 3.
HYPV genome annotation and nucleotide identities for each gene to the most similar strain Eptesipoxvirus (EPTV). The seven conserved genes used for phylogenetic analysis in previous studies [10,12] are presented in bold.
| Gene Name | Putative Product Identity | Start | Stop | +/− | Size | % Id. to EPTV | Orthologs |
|---|---|---|---|---|---|---|---|
| HYPV-1 | Hypothetical protein | 87 | 557 | − | 471 | 58 | EPTV-001 |
| HYPV-2 | Serpin 2 | 1037 | 1552 | − | 516 | 42.5 | EPTV-002 |
| HYPV-3 | Hypothetical protein | 1581 | 2261 | − | 681 | 82.4 | EPTV-003 |
| HYPV-4 | IL-1 receptor-like protein | 2309 | 3316 | − | 1008 | 65.1 | EPTV-004 |
| HYPV-5 | Hypothetical protein | 3356 | 3835 | − | 480 | 88.8 | EPTV-005 |
| HYPV-6 | Tyrosine protein kinase-like protein | 3872 | 4774 | − | 903 | 91.7 | EPTV-006 |
| HYPV-7 | ER-localized apoptosis regulator | 4842 | 5522 | − | 681 | 63.6 | EPTV-007 |
| HYPV-8 | Hypothetical protein | 7002 | 7481 | − | 480 | 80.5 | EPTV-008 |
| HYPV-9 | Ankyrin repeat-containing protein, host range | 8141 | 9826 | − | 1686 | 63.0 | EPTV-010 |
| HYPV-10 | Monoglyceride lipase | 11,053 | 11,913 | − | 861 | 93.1 | EPTV-014 |
| HYPV-11 | Secreted EGF-like growth factor | 12,340 | 12,588 | − | 249 | 62.4 | EPTV-015 |
| HYPV-12 | Anti-apoptotic factor | 12,594 | 13,100 | − | 507 | 65.7 | EPTV-016 |
| HYPV-13 | dUTPase | 13,144 | 13,569 | − | 426 | 87.2 | EPTV-017 |
| HYPV-14 | IFN-inducible protein | 13,597 | 14,004 | − | 408 | 83.7 | EPTV-018 |
| HYPV-15 | Ribonucleotide reductase small subunit | 14,060 | 15,034 | − | 975 | 93.8 | EPTV-019 |
| HYPV-16 | F5L membrane protein | 15,075 | 16,139 | − | 1065 | 68.4 | EPTV-020 |
| HYPV-17 | Cytoplasmic protein | 16,687 | 16,869 | − | 183 | 71.4 | EPTV-023 |
| HYPV-18 | S–S bond formation pathway protein | 17,361 | 18,008 | − | 648 | 92.6 | EPTV-025 |
| HYPV-19 | Ser|Thr protein kinase | 17,998 | 19,314 | − | 1317 | 94.7 | EPTV-026 |
| HYPV-20 | RhoA signaling inhibitor, virus release protein | 19,334 | 20,626 | − | 1293 | 88.0 | EPTV-07 |
| HYPV-21 | EEV maturation protein | 20,659 | 22,602 | − | 1944 | 89.0 | EPTV-028 |
| HYPV-22 | Palmitylated EEV membrane glycoprotein | 22,640 | 23,755 | − | 1116 | 98.9 | EPTV-029 |
| HYPV-23 | Hypothetical protein | 23,781 | 24,008 | − | 228 | 67.1 | EPTV-031 |
| HYPV-24 | Hypothetical protein | 24,050 | 24,250 | − | 201 | 97.0 | Unique to HYPV |
| HYPV-25 | Hypothetical protein | 24,471 | 24,917 | − | 447 | 92.6 | Unique to HYPV |
| HYPV-26 | Conserved non-functional serine recombinase | 24,992 | 25,654 | − | 663 | 78.8 | EPTV-033 |
| HYPV-27 | DNA-binding phosphoprotein | 25,714 | 26,052 | + | 339 | 86.7 | EPTV-034 |
| HYPV-28 | Poly (A) polymerase catalytic subunit | 26,046 | 27,461 | − | 1416 | 92.6 | EPTV-035 |
| HYPV-29 | IEV morphogenesis | 27,478 | 29,676 | − | 2199 | 93.3 | EPTV-036 |
| HYPV-30 | RNA polymerase subunit | 29,733 | 30,455 | − | 723 | 93.8 | EPTV-038 |
| HYPV-31 | IMV protein, virion morphogenesis | 30,760 | 32,463 | + | 1704 | 95.8 | EPTV-039 |
| HYPV-32 | ER-localized membrane protein, virion core protein | 32,490 | 33,302 | + | 813 | 95.6 | EPTV-040 |
| HYPV-33 | DNA polymerase | 33,299 | 36,319 | − | 3021 | 93.8 | EPTV-041 |
| HYPV-34 | Sulfhydryl oxidase (FAD-linked) | 36,352 | 36,642 | + | 291 | 96.9 | EPTV-042 |
| HYPV-35 | Virion core protein | 36,645 | 37,055 | − | 411 | 87.9 | EPTV-043 |
| HYPV-36 | Virulence, modulates Raf|MEK|ERK pathway | 37,039 | 39,117 | − | 2079 | 91.9 | EPTV-044 |
| HYPV-37 | Nonessential glutaredoxin | 39,173 | 39,487 | − | 315 | 91.3 | EPTV-045 |
| HYPV-38 | DNA-binding core protein | 39,613 | 40,545 | − | 933 | 90.3 | EPTV-046 |
| HYPV-39 | IMV membrane protein | 40,546 | 40,767 | − | 222 | 83.6 | EPTV-047 |
| HYPV-40 | ssDNA-binding phosphoprotein | 40,768 | 41,577 | − | 810 | 87.5 | EPTV-048 |
| HYPV-41 | Ribonucleotide reductase large subunit | 41,640 | 43,925 | − | 2286 | 95.2 | EPTV-049 |
| HYPV-42 | IMV protein (VP13) | 43,966 | 44,202 | − | 237 | 88.5 | EPTV-050 |
| HYPV-43 | Telomere-binding protein | 44,220 | 45,371 | − | 1152 | 90.9 | EPTV-051 |
| HYPV-44 | Viral core cysteine proteinase | 45,364 | 46,650 | − | 1287 | 94.6 | EPTV-052 |
| HYPV-45 | RNA-helicase, DExH-NPH-II | 46,656 | 48,686 | + | 2031 | 94.3 | EPTV-053 |
| HYPV-46 | Insulin metalloproteinase-like protein | 48,678 | 50,465 | − | 1788 | 92.3 | EPTV-054 |
| HYPV-47 | Entry|fusion complex component | 50,462 | 50,794 | − | 333 | 97.3 | EPTV-055 |
| HYPV-48 | Late transcription elongation factor (VLTF) | 50,788 | 51,456 | + | 669 | 90.5 | EPTV-056 |
| HYPV-49 | Thioredoxin-like protein | 51,423 | 51,800 | − | 378 | 89.6 | EPTV-057 |
| HYPV-50 | FEN1-like nuclease | 51,803 | 53,140 | + | 1338 | 87.0 | EPTV-058 |
| HYPV-51 | RNA polymerase subunit | 53,142 | 53,333 | + | 192 | 96.8 | EPTV-059 |
| HYPV-52 | NLPc|P60 superfamily protein | 53,337 | 53,870 | + | 534 | 87.7 | EPTV-060 |
| HYPV-53 | Virion structural phosphoprotein, early morphogenesis | 53,836 | 54,933 | − | 1098 | 91.3 | EPTV-061 |
| HYPV-54 | Late transcription factor | 54,962 | 55,744 | + | 783 | 98.5 | EPTV-062 |
| HYPV-55 | Myristylated entry|fusion protein | 55,760 | 56,782 | + | 1023 | 93.8 | EPTV-063 |
| HYPV-56 | Myristylated IMV envelope protein | 56,783 | 57,532 | + | 750 | 96.4 | EPTV-064 |
| HYPV-57 | Crescent membrane|immature virion protein | 57,558 | 57,833 | + | 276 | 84.6 | EPTV-065 |
| HYPV-58 | Internal virion protein | 57,825 | 58,790 | − | 966 | 92.1 | EPTV-066 |
| HYPV-59 | DNA-binding virion protein | 58,815 | 59,573 | + | 759 | 98.4 | EPTV-067 |
| HYPV-60 | IMV protein, entry|fusion | 59,588 | 59,992 | + | 405 | 94.0 | EPTV-068 |
| HYPV-61 | IMV membrane protein, virion morphogenesis | 59,934 | 60,380 | + | 447 | 95.9 | EPTV-069 |
| HYPV-62 | Thymidine kinase | 60,402 | 60,932 | + | 531 | 93.8 | EPTV-070 |
| HYPV-63 | Type I IFN inhibitor | 61,026 | 61,625 | + | 600 | 73.6 | EPTV-071 |
| HYPV-64 | Poly (A) polymerase small subunit | 61,692 | 62,693 | + | 1002 | 94.3 | EPTV-072 |
| HYPV-65 | RNA polymerase subunit (RPO22) | 62,608 | 63,165 | + | 558 | 96.8 | EPTV-073 |
| HYPV-66 | IMV membrane protein, entry|fusion | 63,170 | 63,580 | − | 411 | 94.1 | EPTV-074 |
| HYPV-67 | RNA polymerase subunit (RPO147) | 63,688 | 67,545 | + | 3858 | 98.5 | EPTV-075 |
| HYPV-68 | Tyr|Ser kinase, virus assembly, IFN-gamma inhibitor | 67,542 | 68,060 | − | 519 | 97.7 | EPTV-076 |
| HYPV-69 | Entry|fusion IMV protein | 68,074 | 68,646 | + | 573 | 98.9 | EPTV-077 |
| HYPV-70 | IMV heparin-binding surface protein | 68,654 | 69,667 | − | 1014 | 90.6 | EPTV-078 |
| HYPV-71 | RNA polymerase-associated protein (RAP94) | 69,671 | 72,058 | − | 2388 | 97.5 | EPTV-079 |
| HYPV-72 | Late transcription factor | 72,228 | 72,872 | + | 645 | 71.9 | EPTV-080 |
| HYPV-73 | DNA topoisomerase type I | 72,894 | 73,829 | + | 936 | 93.9 | EPTV-081 |
| HYPV-74 | Crescent membrane|immature virion protein | 73,868 | 74,314 | + | 447 | 88.6 | EPTV-082 |
| HYPV-75 | mRNA capping enzyme large subunit | 74,355 | 76,889 | + | 2535 | 95.7 | EPTV-083 |
| HYPV-76 | Virion core protein | 76,851 | 77,288 | − | 438 | 89.8 | EPTV-084 |
| HYPV-77 | Virion core protein | 77,287 | 78,030 | + | 744 | 83.0 | EPTV-085 |
| HYPV-78 | Uracil DNA glycosylase, DNA pol processivity factor | 78,027 | 78,683 | + | 657 | 96.8 | EPTV-086 |
| HYPV-79 | NTPase, DNA primase | 78,717 | 81,080 | + | 2364 | 96.6 | EPTV-087 |
| HYPV-80 | Early transcription factor small subunit (VETF-s) | 81,077 | 82,984 | + | 1908 | 99.1 | EPTV-088 |
| HYPV-81 | RNA polymerase subunit | 83,017 | 83,532 | + | 516 | 90.1 | EPTV-089 |
| HYPV-82 | Carbonic anhydrase, GAG-binding MV membrane protein | 83,464 | 84,339 | − | 876 | 82.5 | EPTV-090 |
| HYPV-83 | mRNA decapping enzyme | 84,397 | 85,068 | + | 672 | 85.7 | EPTV-091 |
| HYPV-84 | mRNA decapping enzyme | 85,043 | 85,822 | + | 780 | 92.0 | EPTV-092 |
| HYPV-85 | ATPase, NPH1 | 85,796 | 87,703 | − | 1908 | 98.1 | EPTV-093 |
| HYPV-86 | mRNA capping enzyme small subunit | 87,746 | 88,609 | − | 864 | 96.5 | EPTV-094 |
| HYPV-87 | Trimeric virion coat protein | 88,643 | 90,295 | − | 1653 | 94.6 | EPTV-095 |
| HYPV-88 | Late transcription factor (VLTF-2) | 90,321 | 90,776 | − | 456 | 93.4 | EPTV-096 |
| HYPV-89 | Late transcription factor (VLTF-3) | 90,805 | 91,479 | − | 675 | 100.0 | EPTV-097 |
| HYPV-90 | S-S bond formation pathway protein | 91,476 | 91,706 | − | 231 | 93.4 | EPTV-098 |
| HYPV-91 | P4b precursor | 91,726 | 93,726 | − | 2001 | 93.3 | EPTV-099 |
| HYPV-92 | RNA polymerase subunit (RPO19) | 94,462 | 94,992 | + | 531 | 87.5 | EPTV-101 |
| HYPV-93 | Virion morphogenesis core protein | 94,989 | 96,107 | − | 1119 | 94.1 | EPTV-102 |
| HYPV-94 | Early transcription factor large subunit (VETF-L) | 96,131 | 98,275 | − | 2145 | 97.8 | EPTV-103 |
| HYPV-95 | Intermediate transcription factor (VITF-3s) | 98,338 | 99,213 | + | 876 | 94.2 | EPTV-104 |
| HYPV-96 | IMV membrane protein, early morphogenesis | 99,223 | 99,459 | − | 237 | 92.5 | EPTV-105 |
| HYPV-97 | P4a precursor | 99,460 | 102,192 | − | 2733 | 90.5 | EPTV-106 |
| HYPV-98 | Viral membrane formation | 102,207 | 103,142 | + | 936 | 96.1 | EPTV-107 |
| HYPV-99 | Virion core and cleavage processing protein | 103,139 | 103,705 | − | 567 | 76.7 | EPTV-108 |
| HYPV-100 | IMV membrane protein, virion maturation | 103,799 | 104,002 | − | 204 | 71.6 | EPTV-109 |
| HYPV-101 | IMV membrane protein, essential | 104,067 | 104,348 | − | 282 | 96.8 | EPTV-110 |
| HYPV-102 | IMV membrane protein, non-essential | 104,365 | 104,526 | − | 162 | 98.1 | EPTV-111 |
| HYPV-103 | Core protein | 104,516 | 104,809 | − | 294 | 95.9 | EPTV-112 |
| HYPV-104 | Myristylated protein, essential for entry | 104,793 | 105,935 | − | 1143 | 91.8 | EPTV-113 |
| HYPV-105 | IMV membrane protein | 105,936 | 106,526 | − | 591 | 96.9 | EPTV-114 |
| HYPV-106 | DNA helicase, transcript release factor | 106,541 | 107,995 | + | 1455 | 90.1 | EPTV-115 |
| HYPV-107 | Zn finger-like protein, late morphogenesis | 107,967 | 108,188 | − | 222 | 93.2 | EPTV-116 |
| HYPV-108 | IMV membrane protein, entry|fusion | 108,189 | 108,533 | − | 345 | 95.6 | EPTV-117 |
| HYPV-109 | DNA polymerase processivity factor | 108,532 | 109,809 | + | 1278 | 89.4 | EPTV-118 |
| HYPV-110 | Holliday junction resolvase | 109,793 | 110,338 | + | 546 | 91.3 | EPTV-119 |
| HYPV-111 | Intermediate transcription factor (VITF-3L) | 110,335 | 111,495 | + | 1161 | 91.5 | EPTV-120 |
| HYPV-112 | RNA polymerase subunit (RPO132) | 111,492 | 115,010 | + | 3519 | 97.7 | EPTV-121 |
| HYPV-113 | A-type inclusion protein | 114,996 | 117,869 | − | 2874 | 77.4 | EPTV-122 |
| HYPV-114 | P4c precursor | 117,926 | 119,800 | − | 1875 | 82.1 | EPTV-123 |
| HYPV-115 | IMV membrane protein, fusion | 119,856 | 120,206 | − | 351 | 86.2 | EPTV-124 |
| HYPV-116 | IMV membrane protein, entry | 120,207 | 120,623 | − | 417 | 94.2 | EPTV-125 |
| HYPV-117 | RNA polymerase subunit (RPO35) | 120,637 | 121,539 | − | 903 | 94.7 | EPTV-126 |
| HYPV-118 | IMV protein | 121,523 | 121,750 | − | 228 | 92.0 | EPTV-127 |
| HYPV-119 | Hypothetical protein | 121,953 | 122,435 | + | 483 | 80.7 | EPTV-128 |
| HYPV-120 | ATPase|DNA packaging protein | 122,465 | 123,235 | − | 771 | 95.3 | EPTV-129 |
| HYPV-121 | C-type lectin-like EEV membrane phosphoglycoprotein | 123,371 | 123,922 | + | 552 | 77.2 | EPTV-130 |
| HYPV-122 | C-type lectin like IEV|EEV membrane glycoprotein | 123,970 | 124,470 | + | 501 | 91.0 | EPTV-131 |
| HYPV-123 | MHC class II antigen presentation inhibitor | 124,509 | 125,033 | + | 525 | 81.0 | EPTV-132 |
| HYPV-124 | Concanavalin-like precursor | 125,073 | 125,918 | + | 846 | 78.0 | EPTV-133 |
| HYPV-125 | EEV glycoprotein | 125,953 | 126,681 | + | 729 | 68.7 | EPTV-134 |
| HYPV-126 | Hypothetical protein | 126,724 | 127,554 | + | 831 | 79.4 | EPTV-135 |
| HYPV-127 | Hypothetical protein | 127,578 | 127,817 | + | 240 | 68.2 | EPTV-136 |
| HYPV-128 | Truncated CD47-like protein, integral membrane protein | 127,814 | 128,404 | − | 591 | 80.3 | EPTV-137 |
| HYPV-129 | Myristylated protein | 128,422 | 128,829 | + | 408 | 73.3 | EPTV-138 |
| HYPV-130 | Hypothetical protein | 128,826 | 129,587 | + | 762 | 78.3 | EPTV-139 |
| HYPV-131 | Chemokine binding protein | 129,575 | 130,438 | − | 864 | 69.0 | EPTV-140 |
| HYPV-132 | Profilin-like protein, ATI-localized | 130,558 | 130,959 | + | 402 | 98.5 | EPTV-141 |
| HYPV-133 | Hypothetical protein | 130,956 | 131,339 | − | 384 | 76.9 | EPTV-142 |
| HYPV-134 | 3 beta-hydroxysteroid dehydrogenase|delta 5->4 isomerase | 131,948 | 133,015 | + | 1068 | 84.2 | EPTV-144 |
| HYPV-135 | Thymidylate kinase | 133,646 | 134,233 | + | 588 | 85.2 | EPTV-147 |
| HYPV-136 | DNA ligase-like protein | 134,265 | 135,944 | + | 1680 | 87.2 | EPTV-148 |
| HYPV-137 | A52R-like family protein | 137,441 | 138,046 | + | 606 | 77.9 | EPTV-150 |
| HYPV-138 | Hypothetical protein | 138,641 | 139,717 | + | 1077 | 65.2 | EPTV-151 |
| HYPV-139 | Toll|IL-1 receptor-like protein, IL-1, NFkB signaling inhibitor | 139,781 | 140,428 | + | 648 | 89.8 | EPTV-153 |
| HYPV-140 | Hypothetical protein | 140,538 | 140,954 | − | 417 | 70.5 | EPTV-154 |
| HYPV-141 | BTB kelch-domain protein | 141,056 | 142,690 | + | 1635 | 76.8 | EPTV-155 |
| HYPV-142 | Hemagglutinin | 142,737 | 143,477 | + | 741 | 70.2 | EPTV-156 |
| HYPV-143 | Ser|Thr protein kinase | 143,532 | 144,467 | + | 936 | 91.0 | EPTV-157 |
| HYPV-144 | IL-1 receptor antagonist | 144,505 | 145,470 | + | 966 | 71.5 | EPTV-158 |
| HYPV-145 | RING finger protein, host range | 145,503 | 146,378 | + | 876 | 68.4 | EPTV-159 |
| HYPV-146 | Partial schlafen-like protein | 146,424 | 147,011 | + | 588 | 81.1 | EPTV-160 |
| HYPV-147 | EEV type-1 membrane glycoprotein | 147,108 | 147,815 | + | 708 | 75.3 | EPTV-161 |
| HYPV-148 | Anti-apoptotic Bcl-2-like protein | 147,851 | 148,294 | + | 444 | 73.5 | EPTV-162 |
| HYPV-149 | Serpin 1 | 149,551 | 150,555 | + | 1005 | 76.9 | EPTV-164 |
| HYPV-150 | Hypothetical protein | 150,662 | 151,132 | + | 471 | 69.4 | EPTV-165 |
| HYPV-151 | Tyrosine protein kinase-like protein | 151,173 | 152,036 | + | 864 | 77.7 | EPTV-166 |
| HYPV-152 | IL-1 beta-receptor | 152,065 | 153,093 | + | 1029 | 71.9 | EPTV-167 |
| HYPV-153 | Ankyrin repeat protein | 153,125 | 155,104 | + | 1980 | 68.2 | EPTV-168 |
| HYPV-154 | Ankyrin repeat protein | 157,276 | 157,827 | + | 552 | 60.6 | EPTV-169 |
| HYPV-155 | Alpha-amanitin target protein | 158,306 | 159,013 | + | 708 | 82.3 | EPTV-170 |
| HYPV-156 | NFkB inhibitor | 159,072 | 159,752 | + | 681 | 81.1 | EPTV-171 |
| HYPV-157 | Endothelin precursor | 159,796 | 160,020 | + | 225 | 64.9 | EPTV-172 |
| HYPV-158 | NFkB inhibitor | 160,059 | 160,712 | + | 654 | 65.0 | EPTV-173 |
| HYPV-159 | Secreted complement binding protein C3b|C4b | 160,747 | 161,529 | + | 783 | 74.6 | EPTV-174 |
| HYPV-160 | IL-18 binding protein | 162,096 | 162,521 | + | 426 | 83.2 | EPTV-175 |
| HYPV-161 | Ankyrin repeat protein | 164,706 | 166,571 | + | 1866 | 60.3 | EPTV-179 |
+/−: direction of open reading frame (ORF) (“+” (5′–3′) or “−” (3′–5′)).