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. 2019 Jul 15;19:689. doi: 10.1186/s12885-019-5891-y

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© The Author(s). 2019

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 3 — Effects of cisplatin (CDDP) alone or with caffeine (caf), citrate (CA), or caffeine citrate (cafCA) on proliferation of human (HOS) and human fibrosarcoma cells (HT1080). a Representative immunofluorescence microscopic findings of expression of the EdU-positive proliferating cells, including overlay with Hoechst-stained micrographs. Scale bar = 50 μm. The number in the Overlay panels is the rate of EdU-positive cells. b Effects of the various treatments on the numbers of EdU-positive proliferating cells. Data are mean percentages of EdU-positive proliferating cells in five microscopic fields with standard deviations

EdU, 5-ethynyl-2-deoxyuridine.