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. 2019 May 12;6(2):42. doi: 10.3390/vetsci6020042

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© 2019 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Combined FL-RISH and immunohistochemistry (IHC) showing RNA transcripts (green) and Ki67 protein (magenta) in cells of the nucleus pulposus of a mature bovine IVD. DAPI (blue) labels the nucleus. Ki67 indicates active phases of the cell cycle, or cells that are not in G0). Images shown at 63× magnification and zoom factor of three. Glioma-associated oncogene 1 (Gli1); Glioma-associated oncogene 3 (Gli3); Sex determining region Y-box 2 (Sox2); Zinc finger and SCAN (SRE-ZBP, CTfin51, AW-1 and Number 18 cDNA) domain containing transcription factor 10 (Zscan10). Scale bar reflects 10 μm.