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. 2019 May 12;6(2):42. doi: 10.3390/vetsci6020042

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© 2019 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Gaussian mixture model and histograms of data for nine proposed biomarkers in (A) cells of the outer AF and (B) NP of a mature bovine IVD fitted to one or two Gaussian distributions using the expectation-maximization algorithm depending on the normality of the data. Laminin1 (Lam1); Thymocyte differentiation antigen 1 (Thy1); Glioma-associated oncogene 1 (Gli1); Glioma-associated oncogene 3 (Gli3); Notochord (Noto); Tyrosine phosphate receptor type C (Ptprc); Scleraxis (Scx); Sex determining region Y-box 2 (Sox2); Zinc finger and SCAN (SRE-ZBP, CTfin51, AW-1 and Number 18 cDNA) domain containing transcription factor 10 (Zscan10)