Table 1.
Comparison of NP/AF ratio data for nine proposed biomarkers in cells of the outer annulus fibrosus (AF) and nucleus pulposus (NP) of a mature bovine IVD generated through z-proportion test and population averaging comparing data from Li et al [3] using RNA in situ hybridization (AP-RISH) (purple) and this work using Fluorescent RNA in situ hybridization (FL-RISH) (green). Laminin1 (Lam1); Thymocyte differentiation antigen 1 (Thy1); Glioma-associated oncogene 1 (Gli1); Glioma-associated oncogene 3 (Gli3); Notochord (Noto); Tyrosine phosphate receptor type C (Ptprc); Scleraxis (Scx); Sex determining region Y-box 2 (Sox2); Zinc finger and SCAN (SRE-ZBP, CTfin51, AW-1 and Number 18 cDNA) domain containing transcription factor 10 (Zscan10).
| Biomarker | AP-RISH and z-Proportion Test | FL-RISH and z-Proportion Test | FL-RISH and Population Averaging |
|---|---|---|---|
| NP/AF Ratio | NP/AF Ratio | FL in NP/FL in AF Ratio | |
| Lam1 | 0.3 | 0.8 | 0.6 |
| Thy1 | 0.2 | 0.7 | 0.5 |
| Gli1 | 2.2 | 2.0 | 3.8 |
| Gli3 | 1.5 | 2.0 | 3.3 |
| Noto | 4.3 | 2.4 | 2.7 |
| Ptprc | 2.3 | 2.2 | 2.6 |
| Scx | 2.2 | 3.4 | 10.8 |
| Sox2 | 3.2 | 2.2 | 2.1 |
| Zscan10 | 1.6 | 2.0 | 2.5 |