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. 2019 May 24;7(2):44. doi: 10.3390/vaccines7020044

Figure 2.

Figure 2

DNA vaccination produces strong cellular responses in inbred mice. (A) Vaccination schedule to test the immunogenicity of latent proteins in inbred mice. 2 doses of individual or combined latent protein vaccines (vax) were given to groups of 5 BALB/c or C57BL/6 mice two weeks apart, with mouse splenocytes being harvested one week after the final dose (sac). (B) Cellular responses of BALB/c and C57BL/6 mice measured using IFNγ ELISPOT after overnight stimulation with peptide pools. Responses were minimal for LMP1vax, but much larger for EBNA1vax and LMP2Avax. (C) Cellular response measured by flow cytometry. IFNγ staining of cells was measured following their stimulation with latent protein peptides. Pooled EBNA1, LMP1, and LMP2A peptides were used for stimulation. (D) The gating of representative examples of the BALB/c CD8 data is shown. Peptide stimulated splenocytes from a mouse vaccinated with the combination vaccine are shown on the left, and control cells left in media are shown on the right. *p < 0.05, **p < 0.01, ns: not significant.