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. 2019 Apr 12;6(2):32. doi: 10.3390/bioengineering6020032

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© 2019 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Networks of selected miRNAs identified in oviductal extracellular vesicles (oEVs) and potential target genes involved in embryo development. The miRNet online tool was used to represent selected miRNAs (13) (turquoise-blue nodes) based on differential expression and high abundance in oEVs at stage 1 of the bovine estrous cycle (recently ovulated follicle; days 1–4 post-ovulation) and potential target genes (red nodes) enriched in functional categories related to embryo development (green), embryo morphology (blue) and implantation (yellow) in a network. Since the miRNet online tool allows to choose the source/tissue for the list of miRNAs, network (a) represents 11 miRNAs contained in exosomes in the online tool database, with all target genes involved in embryo development. Network (b) represents 3 miRNAs contained in embryos in the online tool database, with all target genes involved in embryo development functions.