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. 2019 Apr 12;6(2):32. doi: 10.3390/bioengineering6020032

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© 2019 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Networks of selected miRNAs identified in oviductal extracellular vesicles (oEVs) and contained in the embryo according to miRNet online tool with potential target genes involved in embryo development. The miRNet online tool was used to represent selected miRNAs (13) (turquoise-blue nodes) based on differential expression and high abundance in oEVs at stage 1 of the bovine estrous cycle (recently ovulated follicle; days 1-4 post-ovulation) and potential target genes (red nodes) enriched in functional categories related to embryo development (green), embryo morphology (blue) and implantation (yellow) in a network. Since the miRNet online tool allows to choose the source/tissue for the list of miRNAs, the network (a) represents three miRNAs contained in embryos according to the online tool database, with all target genes involved in embryo development functions (b).