Treatment consisted of iv injections of PBS, DTX (in Tween 80/ethanol/PEG300), ALK-m/DTX, or ALN-m/DTX at an equivalent dose of 10 mg DTX/kg. Arrows represent iv injections. (A) Quantification of bone metastasis burden based on whole body BLI imaging; (B) Body weight; (C) Kaplan−Meier survival plot; (D) Histopathological analysis of representative kidney, liver, and spleen of mice received different treatments. Data presented as mean ± SEM (n = 8). *p < 0.05 by log-rank (Mantel-Cox) test.