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. Author manuscript; available in PMC: 2019 Dec 1.
Published in final edited form as: Trends Biochem Sci. 2018 Oct 26;43(12):970–983. doi: 10.1016/j.tibs.2018.09.011

Figure 1. Biochemical and Photochemical Reactions Used in Conjunction with Lipid Probes.

Figure 1.

(A) Metabolic labeling of phospholipase D (PLD) activity. PLD functions naturally as a hydrolase that produces phosphatidic acid from phosphatidylcholine, but the addition of low concentrations of exogenous primary alcohols, such as 3-azido-1-propanol, causes PLDs to catalyze a transphosphatidylation reaction to form a functionalized phosphatidyl alcohol. (B) Photochemical reactions that can be used to turn on inactive precursors, including those that can activate photoswitchable (i), photocaged (ii), and photocrosslinkable (iii) lipids. Abbreviation: DAG, diaglycerol.