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. Author manuscript; available in PMC: 2019 Dec 1.
Published in final edited form as: Trends Biochem Sci. 2018 Oct 26;43(12):970–983. doi: 10.1016/j.tibs.2018.09.011

Figure 2. Imaging Phospholipase D (PLD) Activity with Clickable Alcohols via Transphosphatidylation (IMPACT).

Figure 2.

(A) (i) Schematic of IMPACT labeling. Functionalized alcohols are installed into reporter lipids by PLD transphosphatidylation. These unnatural reporter lipids can then be visualized with fluorescent click reagents. (ii) Examples of fluorescent click reagents. (B) IMPACT can be used to visualize intracellular organelle membranes bearing PLD activity. (C) IMPACT can also be used to visualize PLD activity at the single-cell level within a population by fluorescence microscopy (i) or flow cytometry (ii).