KEY RESOURCES TABLE
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Chemicals, Peptides, and Recombinant Proteins | ||
| VX-680 (MK-0457, Tozasertib) | Selleck Chemicals | Cat # S1048 |
| Dasatinib | Cayman Chemical | Cat#11498 |
| Imatinib | Novartis | |
| Axltide | Sigma Aldrich | Cat # 12–516 |
| [Y-32P] ATP | Perkin Elmer | Cat # NEG002A |
| Trichloroacetic acid | Sigma Aldrich | Cat # T4885–500G |
| TEV Protease | Lab generated | N/A |
| Tris-HCl | Sigma Aldrich | Cat # T3253–5KG |
| PMSF | EMD Millipore | Cat # 52332–25GM |
| NP-40 | Fluka | Cat # 74385 |
| β-mercaptoehanol | Sigma Aldrich | Cat # M6250 |
| Sodium Chloride | Fisher Scientific | Cat # BP358–10 |
| NiNTA resin | Qiagen | Cat # 30230 |
| Imidazole | Alfa Aesar | Cat # A10221–2500g |
| Glycerol | Fisher Scientific | Cat # G37–20 |
| PEG 3350 | Sigma Aldrich | Cat # P3015–500G |
| Bis-Tris | Sigma Aldrich | Cat # B9754–100G |
| Ethylene glycol | Sigma Aldrich | Cat # 102466 |
| Ammonium Iodide | Sigma Aldrich | Cat # 03101–100G |
| Kinase tracer 178 | Invitrogen | Cat # PV5593 |
| LanthaScreen Eu-anti-His Antibody | Invitrogen | Cat # PV5596 |
| EGTA | Acros Organics | Cat # 428570100 |
| Brij-35 | Acros Organics | Cat # 329581000 |
| MgCl2 | Sigma Aldrich | Cat # M9272 |
| HEPES | Spectrum | Cat # H1084 |
| Critical Commercial Assays | ||
| Experimental Models: Cell Lines | ||
| Sf9 | ATCC | Cat # CRL-1711 CVCL_0549 |
| Software and Algorithms | ||
| HKL-2000 | HKL Research, Inc |
http://www.hkl-xray.com/hkl-2000 SCR_015547 |
| CCP4 - Phaser | University of Cambridge; Cambridge; United Kingdom |
https://www.phenix-online.org/documentation/reference/phaser.html SCR_014219 |
| Phenix Crystallography Suite | Lawrence Berkeley National Laboratory, University of California at Berkeley; California; USA |
https://www.phenix-online.org/ SCR_014224 |
| Coot | MRC Laboratory of Molecular Biology |
http://www2.mrc-lmb.cam.ac.uk/personal/pemsley/coot/ SCR_014222 |
| Kaleidagraph | Synergy software | |
| Other | ||
| Phosphocellulose paper | Milipore | IPVH 00010 |
| Fluorimeter | Horriba Scientific | Fluoromax 4 |
| Microplatereader | Molecular Devices | SpectraMax M5 |
| Deposited Data | ||
| Structure of DDR1•VX680 | This study | PDB 6BRJ |
| Structure of DDR1•Dasatinib | This study | PDB 6BSD |
| Molecular simulations for DDR1 | This study | Open Science Framework at https://osf.io/4r8x2/. |
| Dendrogram presented in Figure 1A | This study | GitHub at https://github.com/choderalab/DDR1_and_kinase_promiscuity_materials |
| Kinase inhibition data | (Drewry et al., 2017) | |
| Structure of Abl•VX680 | https://www.rcsb.org/ | PDB 2F4J |
| Structure of Abl•Dasatinib | https://www.rcsb.org/ | PDB 2GQG |
| Structure of DDR1•Imatinib | https://www.rcsb.org/ | PDB 4BKJ |
| Structure of CSF1R | https://www.rcsb.org/ | PDB 3KRL |
| Structure of CSF1R | https://www.rcsb.org/ | PDB 2IOV |
| Structure of c-Kit | https://www.rcsb.org/ | PDB IT45 |
| Structure of c-Kit | https://www.rcsb.org/ | PDB IT46 |
| Structure of DDR1 | https://www.rcsb.org/ | PDB 5FDP |
| Structure of DDR1 | https://www.rcsb.org/ | PDB 5BVO |
| Structure of PDGFRA | https://www.rcsb.org/ | PDB5GRN |
| Structure of DDR1•ponatinib | https://www.rcsb.org/ | PDB 3ZOS |
| Structure of Abl•ponatinib | https://www.rcsb.org/ | PDB 3OXZ |
| Structure of Abl•imatinib | https://www.rcsb.org/ | PDB 2HYY |