Figure 3.

Cell-free production kinetics of colicin E1. (A) Yields of soluble colicin E1 produced in CFPS over time. Yields were determined by incorporation of radioactive ¹⁴C-leucine and normalized to the maximum yield observed after 3 h incubation. (B) Viability of K361 cells (initial cell density 5 × 10⁷ CFU/mL) upon treatment with 250 ng/mL of soluble cell-free produced colicin E1 at 37 °C 220 rpm for 1 h. Error bars indicate standard deviation from two independent cultures with three plating replicates each. *** represents significant difference compared to 0 h sample under the same media conditions with p-value < 0.0001.