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. 2019 Apr 16;2(2):30. doi: 10.3390/mps2020030

Table 2.

Primer sequences used for the generation of eYFP c-terminally fused to Mel-WNT3a (Mel-WNT3a-eYFP).

Primer Primer Sequence 5′ → 3′
Gene-specific reverse (R) primer
X-Mel-WNT3aOpt-oeXFP-R CTT GCT CAC CTC TAG ACA GGG CAC CTT TCC AGC G
eYFP primer
X-eXFP-SR TTG CGG ATG AGA CCA GGC AGA CTT GTA CAG CTC GTC CAT GC
oe-eXFP-F TGT CTA GAG GTG AGC AAG GGC GA
Adapter primer
C-SII TGT CTA GAG GTG AGC AAG GGC GA