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. 2019 Jun 7;2(2):48. doi: 10.3390/mps2020048

Figure 1.

Figure 1

Schematic diagram illustrating the experimental protocol for the expression and isolation of recombinant human Aβ(M1-42) peptide from competent E. coli. The protocol can be divided into five main parts: Part 1. Isolation of the pET-Sac-Aβ(M1-42) plasmid from the glycerol stock; Part 2. Transformation of BL21(DE3)pLysS and Rosetta(DE3)pLysS competent cells with the isolated pET-Sac-Aβ(M1-42) plasmid; Part 3. Expression of the Aβ(M1-42) peptide in 1 L liquid LB culture; Part 4. Harvesting and lysis of the cells using a probe sonicator followed by resuspension of the cell lysate in 8 M urea; and Part 5. Purification of the Aβ(M1-42) peptide with preparative HPLC.