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. 2019 Jun 7;2(2):48. doi: 10.3390/mps2020048

Figure 3.

Figure 3

High-performance liquid chromatography (HPLC) set-up. A. The water bath containing filtered reverse osmosis water is heated to around 80 °C. B. Both columns are completely submerged in the water bath. One end of the guard column attached to the injection valve with metal tubing and the other end of the guard column attached to the inlet of the primary C18 100 Å 5 μm 10 mm × 250 mm preparative column. The primary column is fed into the Combiflash. C. 5 mL injection loop. D. Collection of Aβ(M1-42) fractions eluted at 26 min.