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. 2019 Jun 7;2(2):48. doi: 10.3390/mps2020048

Table 2.

Solvent gradient for peptide purification.

% Solvent A 1 % Solvent B 2 Elapsed Time (min)
90 10 0
90 10 9
5 95 19
5 95 27

1 Solvent A = H2O with 0.1% TFA 2 Solvent B = Acetonitrile with 0.1% TFA.