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PCR amplifications using DNA extracted with the optimized protocol from L. longipalpis (Argentina) and L. intermedia. DNA extracts were evaluated with an internal control PCR (using primers 44F/44R) and were diluted (1:5). Color-coding coincides with that used for Table A1. 1: MW (pZero2/HaeII); 2: positive control; 3–4: L. longipalpis (Posadas, Misiones, Argentina); 5–6: L. intermedia (Tancredo Neves, Bahia, Brazil); 7: negative control.
