Figure 3.

GSK-3β inhibits Ca²⁺ influx through P/Q-type calcium channels. A, Calcium images were recorded as described in Figure 2, except that the P/Q-type (Aga) or N-type (CgTx) Ca²⁺ channel inhibitors were added 30 min before recording. The Fluo3Am increment (ΔF) were calculated by subtracting the preimage from the postimage of Fluo3Am and transferred into the fluorescence gradient images using Image Pro Plus 4.5 and LSM510. Scale bar, 20 μm. B, C, Quantitative analysis of the relative increment. ΔF/Δt (F, fluorescence intensity; t, time). The results show that only inhibition of P/Q-type calcium channel retards the dnGSK-3β-induced calcium influx. Mean ± SD, ANOVA, Student's t test; **p < 0.01 untransfected versus dnGSK-3β-transfected; ^## p < 0.01 DMSO versus inhibitors in untransfected neurons; ^$$ p < 0.01 DMSO versus inhibitors in dnGSK-3β-transfected neurons (n = 6).