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. 2010 Mar 10;30(10):3624–3633. doi: 10.1523/JNEUROSCI.5223-09.2010

Figure 4.

Figure 4.

GSK-3β phosphorylates P/Q-type calcium channels. A, In vitro phosphorylation of LII-III by GSK-3β: the purified LII-III (20 μg) was incubated with GSK-3β in the presence of 100 μm [γ-32P]ATP for autoradiography and Western blotting with the antibodies against GSK-3β, LII-III, pThr, and phosphoserine (pSer), respectively (N= rat brain cortex extract). B, Phosphorylation of LII-III by GSK-3 in synaptosome: the purified synaptosome (P2) from rat brain hippocampi was incubated with DMSO, Wort, or SB, or Wort plus SB for 30 min, then the suspension was immunoprecipitated with anti-LII-III antibody and the supernatant (S), and the pellets were probed by anti-pThr or anti-LII-III (M = marker). X = 20. Note that GSK-3β can phosphorylate P/Q-type calcium channel at Thr residues.