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Glia cultured from the ventrolateral medulla express immunolabeling for the P2Y1R. Epifluorescence images of a glial culture showing immunolabeling for the glial marker S100β (monoclonal anti-S-100β-subunit antibody with Alexa Fluor 488 donkey anti-mouse IgG, A) and the P2Y1R (rabbit anti-P2Y1 with Alexa Fluor 594 donkey anti-rabbit IgG, B). C, Hoechst staining of cell nuclei. D, The overlay of A–C.
