Figure 6.

Inhibition of APP aggresome formation by suppression of HRD1 expression. a, SH-SY5Y cells stably expressing APP-FLAG were transiently transfected with NC (top) or HRD1 (bottom) siRNA. The cells were subjected to immunofluorescence staining with anti-FLAG (green) and anti-cleaved caspase-3 (red) antibodies. Scale bars, 100 μm. b, Statistical analysis of Figure 6a. The percentage of apoptotic cells was counted in three different areas. The results are expressed as mean ± SEM (n = 3). Statistical analysis was performed with ANOVA followed by Bonferroni correction (*p < 0.05, **p < 0.01). c, Localization of APP and γ-tubulin protein. d, Localization of HRD1 and γ-tubulin. SH-SY5Y cells were treated with 5 μm MG132 for 12 h and then subjected to immunofluorescence staining using APP (C-terminal), HRD1 (Otsuka), and γ-tubulin antibodies. Scale bars, 20 μm.