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. 2010 Mar 17;30(11):4120–4131. doi: 10.1523/JNEUROSCI.5185-09.2010

Figure 6.

Figure 6.

Pals1 is required for radial and longitudinal extension of the myelin sheath. A, Cells infected with pals1 shRNA virus express Dsred2 or GFP (white) and show an abnormally thin diameter (right lower panel, 2.6 μm diameter for 433.1 μm length) and a reduced length (left lower panel) in comparison to a cell infected with control shRNA vector (upper panel, 8.4 μm diameter for 497.3 μm). The inset shows the detail of the left node of Ranvier of the pals1-silenced cell. Scale bars, 50 μm. B, Short cells infected with pals1 shRNA virus express Dsred2 (red) and Krox20 (green) (upper panels), GFP (green), and E-cadherin (red) (lower panels). Scale bars, 5 μm. C, Graph displaying cell diameter versus length of mSCs infected with viruses expressing control shRNA (gray squares) and pals1 shRNA (black squares). Lines show linear regressions lines for control shRNA (1) and pals1 shRNA (2). Tissue was from mice injected 3–4 d after birth and analyzed 2 months later.