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. 2010 Mar 17;30(11):4062–4071. doi: 10.1523/JNEUROSCI.2964-09.2010

Figure 4.

Figure 4.

Transient transfection assays define a cis-regulator in mouse gat1 gene promoter. A, Schematic structure of the luciferase constructs used in this study. The boundaries of the promoter constructs are defined relative to the transcription initiation site and are indicated on the left. Independent constructs were transfected into NIH 3T3 or Neuro 2a cells. Luciferase activity was normalized to Renilla luciferase activity encoded by cotransfected control plasmid pRL-SV40 and then normalized to that of −5377/luc construct. B, In the −5377m/luc or −1090∼+52m/luc construct, a 46 bp fragment, from −333 to −288, is indicated by an open rectangle and was deleted. Independent constructs were transfected into NIH 3T3, Neuro 2a cells, primary cortical neurons, or NSCs. Luciferase activity was normalized to Renilla luciferase activity encoded by cotransfected control plasmid, pRL-SV40, or pRL-TK. The promoter activity was normalized to −5377/luc or −1090∼+52m/luc. Induction of promoter activity in different cell lines is indicated as fold increase. Results are shown as the mean ± SD for three independent experiments (n = 3 in each independent experiment).