Figure 6.
Quantitative analysis of LacZ or GAT1 immunopositive cells in cerebral cortex of gat1(5.7)lacz and gat1(5.7m)lacz mice. A, Schematic representation of a sagittal brain section showing image locations. B, Representative images of LacZ and GAT1 immunopositive signal on sagittal brain sections from gat1(5.7)lacz or gat1(5.7m)lacz mice. C, D, The percentage of LacZ-expressing cells in GAT1-expressing cells or the percentage of GAT1-expressing cells in LacZ-expressing cells of region I (C) or region II (D). E, F, Intensity of LacZ-immunoreactive signal in all cells or in GAT1-expressing cells of region I (E) or region II (F). Scale bars: A, 1 mm; B, 100 μm. Data represent mean ± SE (n ≥ 5 mice per line). *p < 0.05 significantly different from gat1(5.7)lacz group (ANOVA followed by Bonferroni correction). DAPI, 4′,6′-Diamidino-2-phenylindole dihydrochloride.