Figure 2.

Progressive reduction in GABAergic synaptic puncta of CSP-α KO hippocampal neuron cultures. Confocal images at two different magnifications of WT and CSP-α KO hippocampal neuron cultures at 11 DIV labeled with anti-synaptobrevin 2/VAMP2 (green; asterisks) and anti-VGAT (white and red) antibodies identify GABAergic synaptic puncta at colocalization points (arrowheads) that coexist with non-GABAergic puncta (asterisks). A, At 11 DIV, the percentage of GABAergic puncta is similar in WT and CSP-α KO cultures. B, At 30 DIV, the percentage of GABAergic puncta is significantly reduced in CSP-α KO (21.6%) compared with WT cultures (42.5%).