Figure 6.

Increase in Thioflavin S-positive amyloid-β plaques in reln/app mice in neocortex and hippocampal formation. Double immunofluorescence using anti-Aβ (6E10; red) and anti-α1-syntrophin (αSyn; blue) antibodies combined with Thioflavin S counterstaining (green) were performed to investigate the fibrillary amyloid-β plaque load in both neuropil and cerebral vasculature in 9-month-old app (A) and reln/app mice (B). Note the increase in Thioflavin S signals concomitantly with the anti-Aβ immunoreactivity in the outer layers of the neocortex. C, Densitometric analysis of the brightness and surface area covered by Thioflavin S-positive signals in the neocortical layers I–VI (Ctx), lateral entorhinal cortex (EC), and dorsal and ventral hippocampus (Hip). D, Quantification of the pixel overlap between the green Thioflavin S-positive signals and red anti-Aβ immunoreactive staining, averaged from measurements of eight images (512 × 512 pixel in size) per animal and brain region (n = 4 per genotype) acquired in the areas indicated. Values are given as mean ± SEM. *p < 0.05, statistical significance based on Mann–Whitney U test. Scale bar, 50 μm.