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. 2019 Jul 9;10:801. doi: 10.3389/fpls.2019.00801

FIGURE 3.

FIGURE 3

PDB ID of Super positioned structures: 5FW2, 5FW3, 5FW1, 5B2S, 5B2R, and SB2T. The reported mutations are VQR: D1135V, R1335Q and T1337R, EQR: D1135E, R1335Q and T1337R, VRER: D1135V, G1218R, R1335E and T1337R. In total, 12 mutations screened from Anders et al., and Hirano et al., reported structure in which 6 synonymous syntactic parsers from PDB and 6 non-synonymous mutations. sSNP and nsSNP are significant roles in choosing guide RNA specificity. The screened mutated regions are used to choose Cas9 variant for genome editing in crops.