Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.
Modified PAM sequences among various variants of SpCas9 and their respective mutations.
| SpCas9 variant | Mutations (relative to SpCas9) | PAM sequence |
| D1135E variant | D1135E | NGG |
| VQR variant | D1135V, R1335Q and T1337R | NGAN or NGNG |
| EQR variant | D1135E, R1335Q and T1337R | NGAG |
| VRER variant | D1135V, G1218R, R1335E and T1337R | NGCG |
