Figure 1.
Lhx6-EGFP is reliably expressed in fast-spiking perisomatic-targeting PV+ cells. A, Confocal image stack from a horizontal section (50 μm) through the DG of a juvenile Lhx6-EGFP mouse. Note the high density of Lhx6-EGFP-positive somata at the gcl–hilus border (arrows). Inset, Confocal image demonstrating colocalization of EGFP and Lhx6 detected with a primary antibody and visualized using a secondary antibody conjugated to Cy3. Scale bar, 20 μm. B, Left, A single Lhx6-EGFP-positive neuron was filled with biocytin during whole-cell recording and visualized subsequently. The dense axonal arborizations in the gcl (arrows) identify this neuron as a perisomatic-targeting cell. The selected somatic area (red box) is shown on the right at higher magnification. Inset, Perisomatic-targeting cells had a typical fast-spiking non-adapting discharge pattern (600 pA, 1 s; Vhold −70 mV). Right, Confocal image stack of the same cell stained against parvalbumin, a characteristic marker for perisomatic-targeting cells. Scale bar, 10 μm. ml, Molecular layer.