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Immunoisolation of SVs using VGLUT-1- and VGAT-specific antibodies. Immunoblot analysis shows that VGLUT-1-isolated vesicles are largely depleted for VGAT vesicles (top panel) and VGAT-isolated vesicles are largely depleted of VGLUT-1 vesicles (bottom panel). LP2, Enriched synaptic vesicle fraction; IP, immunoprecipitated sample; FT, flow through (supernatant) after IP.
