Figure 7.

Association of MAL2 with glutamatergic nerve terminals in primary cultures of hippocampal neurons (see legend to Fig. 4 for details). A, Immunostaining for MAL2 (red channel) is observed in the soma, axons and dendrites of both glutamatergic (VGLUT-1, green stain) and GABAergic (VGAT, green strain) neurons. Magnified views reveals punctuate staining for MAL2 as expected for a SV protein (A, B) with high degree of colocalization with VGLUT-1 in contrary to VGAT. C, Correlative line-scans of synaptic boutons demonstrate selectivity of MAL2 for VGLUT-1-positive nerve terminals.