Figure 6.
Glycine can be released by RCs in E12.5 embryonic spinal cords. A, Double immunostaining showing glycine-ir RCs in control conditions (325 mosmol/kg H2O). Anti-nestin (green), anti-glycine (red), and merged images are shown. Arrows, Examples of double-stained RC end feet. B, Exposing the embryonic SC to 200 mosmol/kg H2O solution during 1 h in the presence of GlyT blockers abolished the glycine labeling in nestin-ir RCs. Images are single 0.3-μm-thick confocal images. C, Variation in the proportion of double-stained glycine/nestin RC fibers according to various pharmacological treatments. Note that the simultaneous application of SKF-96365 and flufenamic acid partially reversed the effect induced by a hypotonic solution in the presence of GlyT blockers. Statistical tests are one-way ANOVA followed by a Dunnett's multiple comparison (test vs control or hypotonic/GlyT blockers: *p < 0.05; **p < 0.01). The number of sections analyzed in each condition is indicated above the bars. Error bars indicate SEM.