Figure 3.

LGI1 and Nogo66 compete for the same binding site on NgR1. A, Fc or Fc-NgR1 fusion proteins were bound to protein A beads, extensively washed, and then incubated with purified LGI1. After extensive washing, proteins bound to beads were eluted and analyzed by immunoblot, as indicated. Purified LGI1 bound Fc-NgR1 but not Fc alone. The position of Fc-NgR1 is indicated by a single arrowhead, Fc is indicated by a double arrowhead. B, Fc-NgR1 fusion proteins bound to protein A beads were incubated with purified LGI1 in the presence of AP alone, AP-LGI1 or AP-Nogo66. After extensive washing, proteins bound to the protein A beads were eluted and analyzed by immunoblot, as indicated. C, P5 rat DRG neurons were grown on GST or GST-Nogo66 in the absence or presence of purified LGI1 (5 nm). For C, statistical differences between groups was established using Student's t tests (*p < 0.05, **p < 0.001).