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. 2010 May 12;30(19):6620–6634. doi: 10.1523/JNEUROSCI.5778-09.2010

Figure 6.

Figure 6.

The MuSK binding domain of Erbin is encoded by exon 21. a, Drawing of full-length human Erbin-v2 and small fragments representing parts of Erbin. Each of these recombinant fragments was expressed and purified as GST fusion for pull-down assays. GST fusion proteins were incubated together with HEK293 cell extracts containing myc-tagged MuSK2xwt. b, Western blot of GST pull-down experiments immunoassayed with an anti-myc antibody to detect myc-tagged MuSK2xwt. Note that the shortest Erbin part able to pull down MuSK2xwt spans from amino acid residue 1145 to 1229. c, Western blot images of GST pull-down experiments, like in b, are presented. Here, Erbin mutants were designed such that they helped to define exactly the MuSK binding domain of Erbin. Note that amino acid residues encoded by exon 23 were completely unable to interact with MuSK2xwt. d, RT-PCRs were performed to find out whether either C2C12 cell nuclei or extrasynaptic or synaptic nuclei of diaphragm muscle fibers transcribe differently spliced Erbin variants. MB, Myoblast; MT, myotube.