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. 2010 Apr 28;30(17):5830–5842. doi: 10.1523/JNEUROSCI.0119-10.2010

Figure 1.

Figure 1.

Synaptic activity regulates eEF2 phosphorylation in dendrites. A, Western blots of neurons treated with TTX or bicuculline for 24 or 48 h and then solubilized in SDS sample buffer. B, Band intensity of P-eEF2, normalized against total eEF2. The vertical axis shows the mean fold increase over no treatment (NT). Error bars are SEMs; at least four independent experiments were performed per condition. *p < 0.05 versus NT; #p < 0.05 versus TTX samples (ANOVA, Tukey post hoc test). C, Hippocampal neurons untreated (NT), treated with TTX, or bicuculline (Bic) at DIV16, fixed at DIV18, and immunostained to reveal P-eEF2 and MAP2. TTX and Bic treatments modified dendritic but not somatic eEF2 phosphorylation. D, P-eEF2 signals in somatic and dendritic regions expressed as mean pixel intensity (error bars SEM); >15 neurons from three independent experiments were used for condition (soma: NT: 44.8 ± 3.50; TTX: 36.9 ± 3.09; Bic: 47.1 ± 2.53; dendrite: NT: 36.2 ± 4.63; TTX: 13.7 ± 1.83, Bic: 65.2 ± 6.83). *p < 0.01 versus untreated neurons, #p < 0.01 versus TTX-treated neurons (ANOVA, Tukey test). Scale bar, 20 μm for low-magnification images; Scale bar, 10 μm for high-magnification images.