Figure 9.

Norrin induces the expression of neurotrophic growth factors in cultured Müller cells via the Wnt/β-catenin pathway. A, Quantitative real-time RT-PCR for neurotrophic growth factors in RNA of Müller cells after incubation for 7 h with DKK-1(100 ng/ml) and/or Norrin (40 ng/ml), and in untreated controls (mean ± SEM; n ≥ 4; *p < 0.05; **p < 0.01). B, C, Dot blot analyses of proteins from cell culture medium of Müller cells for FGF2 (B) and CNTF (C) after incubation for 24 h with Norrin (40 ng/ml) and in untreated controls.