Figure 4.

Effects of chronic undernutrition along puberty on hypothalamic expression of NUCB2. In A, hypothalamic mRNA levels of NUCB2 in pubertal OVX female rats, either fed ad libitum [Co-ad lib (Con)] or subjected to 25% reduction in daily food intake along puberty [Undernutrition (UN)]. Representative RT-PCR assays and semiQ data (mean ± SEM; n ≥ 5 independent determinations, done in duplicate) are presented. In B, hypothalamic NUCB2 protein levels in fed and 25% food-restricted conditions are shown, with representative Western blots and semiQ data being presented. **p ≤ 0.01 versus control group (Student's t test).