The inhibition of the NR1 subunit does not affect the recall of memory. A, Two days after conditioning, animals were given injections of dsRNA (gray, dsNEG; white, dsNR1). One day later, the NR1 expression levels were evaluated in the MB and AL regions. The NR1 levels were evaluated by quantitative Western blot, using AmNR1. Each column represents the mean ± SEM of n measurements as indicated by the numbers on the bars. The asterisk indicates a significant reduction (*p < 0.05, Student's t test). B, Animals were subjected to three CS–US pairings (A1–A3). Two days after conditioning, they were given injections of dsRNA, and memory was retrieved on day 3. The number of animals is indicated in the inset. PER, Percentage of animals that showed a PER during the CS presentation.