PPK28 is sufficient to confer water sensitivity. A, Water-elicited spiking (1 mm KCl) in the intermediate-type sensilla (I7 or I8) of UAS-ppk28, Gr66a-GAL4, and Gr66a-GAL4>UAS-ppk28 flies. B, Quantification and statistics of results in A (***p < 1 × 10−5, t test, n = 9–21 sensilla). C, Inhibition of water-elicited spiking in Gr66a-GAL4>UAS-ppk28 flies by 30 mm TCC or 100 μm amiloride.