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Promoter activity of SNCA(−1524/−189) in HeLa cells. In vitro methylation of the reporter construct SNCA(−1524/−189) reduced relative luciferase activity by 98.5% (**p ≤ 0.01, one-way ANOVA). Absence of SssI or SAM from the in vitro methylation reaction minimized reduction. Ctrl, Construct with the inverted insert.
