Figure 2.

Robust and persistent rAd-driven transgene expression in the SNpc over time. A, Representative photomicrographs showing GFP and LRRK2 overexpression, revealed by anti-GFP and anti-FLAG immunostaining, respectively, in retrogradely transduced neurons of the SNpc of animals killed at 10, 21, and 42 d after injection. All animals at each time point displayed robust transgene expression. B, High-magnification photomicrographs of anti-FLAG immunostainings in the SNpc of rAd-WT-LRRK2- and rAd-G2019S-LRRK2-injected animals at 10 d. Overexpressed LRRK2 localized to the cytoplasm and neuritic processes of transduced nigral neurons. No visible differences in cellular localization were observed between the wild-type and mutant form of overexpressed LRRK2. Scale bars: A, 200 μm; B, 50 μm.