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. 2010 Jan 13;30(2):749–759. doi: 10.1523/JNEUROSCI.2343-09.2010

Figure 3.

Figure 3.

GABAB receptor activation can increase Akt phosphorylation. A, Time course of Akt activation in CGNs after incubation with GABA (100 μm), baclofen (100 μm), or CGP7930 (50 μm). Representative immunoblots were used to quantify phosphorylated Akt levels (pAkt). *p < 0.05, **p < 0.01, ***p < 0.001, versus basal level for baclofen dose–response. B, Response of baclofen or CGP7930-induced Akt phosphorylation after 10 min incubation. Immunoblots were used to quantify pAkt levels. *p < 0.05, **p < 0.01, versus basal levels. C, D, Cells were treated with CGP54626, bicuculline, or TPMPA at 10 μm for 20 min before the stimulation of baclofen/GABA at 100 μm (10 min). Akt phosphorylation was measured by quantifying pAkt from immunoblots. ***p < 0.001, versus basal levels. +++p < 0.001; ns, nonsignificance, versus treated with baclofen, GABA, or CGP7930 in the absence of antagonist. The blots shown are representative of three separate experiments.