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. 2010 Jan 13;30(2):749–759. doi: 10.1523/JNEUROSCI.2343-09.2010

Figure 6.

Figure 6.

Activation of GABAB receptor transactivated IGF-1R. A, Time course of baclofen- (100 μm) and CGP7930- (50 μm) induced IGF-1R phosphorylation in CGNs. B, Effects of pretreatment with PTX (200 ng/ml) or CGP54626 (10 μm) on IGF-1R phosphorylation induced by baclofen (100 μm) or CGP7930 (50 μm) for 10 min, and representative immunoblots. C, Western blot analysis of IGF-1R coimmunoprecipitated from CGNs with GABAB1 antibody. The membrane was immunoblotted by anti-IGF-1R antibodies. The total lysate (input) was analyzed. D, Effect of the neutralizing antibody anti-IGF-1R (αIR3; 2 μg/ml) on baclofen- (100 μm) or IGF-1 (10 ng/ml) induced IGF-1R phosphorylation for 10 min. For all IGF-IR phosphorylation results, representative immunoblots are shown under the quantified data of pIGF-IR analyzed from separate experiments (mean ± SEM; n = 3–5). For all results, *p < 0.05, **p < 0.01, and ***p < 0.001, versus the basal levels, and +p < 0.05, ++p < 0.01, +++p < 0.001, and ns, nonsignificance, versus treatment with baclofen or CGP7930 in the absence of CGP54626, PTX, IGF-1, or mAb αIR3.